Testing for Virucidal Activity

What is a Virucidal Efficacy Test?

A virucidal efficacy test evaluates the virucidal effect of a biocidal product or disinfectant on viruses. These tests scientifically demonstrate that the product is effective not only against bacteria, but also against enveloped or non-enveloped viruses.

Why is this important?

• Critical for Public Health: Particularly in healthcare facilities, the food industry, and household hygiene, products that neutralize viruses are preferred.
• Legal Obligation: Under the European Biocidal Products Regulation (BPR), virucidal activity may be required for product authorization.
• Consumer Confidence: Claims such as “99.99% effective against viruses” can only be substantiated through these tests.
• Market Strength in Times of Crisis: During pandemics, products with virucidal authorization are quickly favored.

Applied Standards and Test Methods

Standard EN 14476: This is the most widely used test for virucidal efficacy in Europe.

Test Viruses:

• Poliovirus
• Adenovirus
• Norovirus
• Murine Norovirus (MNV)
• Vaccinia virus (enveloped virus model)
• SARS-CoV-2 (optional or special test conditions)

ASTM E1052: A test method developed in the USA that measures virus reduction in liquid products.

DVV/RKI Guideline (Germany): Official criteria for disinfectants used in the medical sector in Germany.

How is the Test Performed?

1. The test viruses are cultured.
2. The product is mixed with the viruses at specific concentrations and for defined periods.
3. At the end of the contact time, the reduction in viral load is measured.
4. A reduction of ≥4 log10 (i.e., 99.99% inactivation) is considered a successful result.