What is the Chromosomal Aberration Test in Mammalian Bone Marrow (OECD TG 475)?
Genotoxicological studies are a system for assessing the potential of a substance to damage DNA or chromosomes. In this context, OECD TG 475, i.e., the test for chromosomal aberrations in mammalian bone marrow, is a widely used standard test, particularly for determining chromosomal damage in vivo.
Purpose and Significance of the Test
This test is used to determine whether a chemical substance causes structural chromosomal aberrations in mammalian bone marrow cells. The data obtained play an important role in understanding the genetic toxicity potential of a substance and thus the risks to human health. The test is particularly used in the development of pharmaceuticals, pesticides, cosmetics, or industrial chemicals.
Application Process under OECD TG 475
The chromosomal aberration test in mammalian bone marrow is usually performed on rodents. The test process can be summarized as follows:
- The test substance is administered to the animal at an appropriate dose.
- After a certain period of time after administration, bone marrow cells are collected.
- The cells are prepared for microscopic examination.
- The chromosomes are examined for structural changes (breaks, fragmentation, rearrangement, etc.).
- The results are compared with those of a control group.
Why are in-vivo tests important?
In-vitro tests conducted in the lab do provide valuable information, but they cannot fully reflect the complex biological processes in a living organism. Therefore, in-vivo tests like OECD 475 are indispensable for realistic and reliable risk assessments.
What do we offer as Nanolab Laboratory?
As Nanolab, we reliably, quickly, and transparently conduct chromosomal aberration tests according to the international standards of OECD TG 475. With our expert team and modern laboratory infrastructure, we analyze the genetic safety of your products with the utmost care.
In which areas is it used?
- Drug safety
- Analysis of the toxicity of chemical substances
- Safety assessment of plant protection products and
- Biocide products
- Development processes for cosmetic products
- Environmental compatibility tests
Determining the potential of a substance to cause structural changes in genetic material is a crucial step in protecting human health and ensuring product safety. For this reason, the chromosomal aberration test in mammalian bone marrow according to international regulations is a mandatory or recommended test.