{"id":4559,"date":"2026-10-12T20:04:01","date_gmt":"2026-10-12T20:04:01","guid":{"rendered":"https:\/\/nano-test.de\/?p=4559"},"modified":"2026-10-12T20:05:12","modified_gmt":"2026-10-12T20:05:12","slug":"hautreizungstest-durch-biozide-oecd-404","status":"publish","type":"post","link":"https:\/\/nano-test.de\/en\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/","title":{"rendered":"Skin irritation test by biocides | OECD 404"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"4559\" class=\"elementor elementor-4559\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3479c652 ts-col-stretched-none ts-bg-color-over-image elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3479c652\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-537b5195 ts-bg-color-over-image\" data-id=\"537b5195\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-79ebcdc8 elementor-widget elementor-widget-text-editor\" data-id=\"79ebcdc8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #000000;\">Biozidprodukte sind Zubereitungen, die aktive chemische Stoffe enthalten und in einem breiten Spektrum eingesetzt werden \u2013 von Desinfektionsmitteln \u00fcber Sch\u00e4dlingsbek\u00e4mpfungsmittel bis hin zu Holzschutzmitteln und Antifouling-Farben. Je nach Verwendungszweck k\u00f6nnen diese Produkte direkt oder indirekt mit der menschlichen Haut in Kontakt kommen. Insbesondere Produkte wie Handdesinfektionsmittel, Oberfl\u00e4chenreiniger und Pflanzenschutzmittel k\u00f6nnen potenziell hautreizend wirken. Daher ist der <strong>Hautirritationstest f\u00fcr Biozidprodukte<\/strong> sowohl im Hinblick auf die Sicherheit der Mitarbeiter als auch auf die Gesundheit der Endverbraucher eine obligatorische Bewertung.<\/span><\/p><p><span style=\"color: #000000;\">Under the Biocide Regulation (BPR - 528\/2012) of the European Union, the skin irritation potential of a biocidal product must be scientifically evaluated in order to allow it. In Turkey, similar requirements were adopted into national legislation by the Ministry of Health issued by the Ministry of Health. These evaluations must be carried out according to internationally recognized methods and according to the GLP principles (good laboratory practice).<\/span><\/p><p><span style=\"color: #000000;\"><!-- H2-&#220;berschrift --><\/span><\/p><h2><span style=\"color: #000000;\">The importance of skin irritation in biocidal products<\/span><\/h2><p><span style=\"color: #000000;\">Skin irritation is a local inflammatory reaction triggered by a substance contact with the skin and not due to the immune system. It is characterized by redness (erythema), swelling (edema), drought, cracks and sometimes necrosis. In the case of biocidal products, this risk is particularly critical in the following cases:<\/span><\/p><ul><li><span style=\"color: #000000;\"><strong>Berufliche Exposition:<\/strong> Mitarbeiter, die mit Pflanzenschutzmitteln, industriellen Desinfektionsmitteln und Holzschutzmitteln arbeiten, sind langanhaltendem und wiederholtem Hautkontakt ausgesetzt.<\/span><\/li><li><span style=\"color: #000000;\"><strong>Verbrauchereinsatz:<\/strong> Haushaltsdesinfektionsmittel, Handhygieneprodukte und Sch\u00e4dlingsbek\u00e4mpfungssprays bergen das Risiko eines direkten Hautkontakts.<\/span><\/li><li><span style=\"color: #000000;\"><strong>Versehentlicher Kontakt:<\/strong> Akute Expositionen, die durch unsachgem\u00e4\u00dfen Gebrauch oder das Versch\u00fctten der Produkte entstehen k\u00f6nnen.<\/span><\/li><li><span style=\"color: #000000;\"><strong>Formulierungsbestandteile:<\/strong> Bestandteile wie Tenside, L\u00f6sungsmittel, S\u00e4uren und Basen k\u00f6nnen das Reizungsrisiko erh\u00f6hen.<\/span><\/li><\/ul><p><span style=\"color: #000000;\">The early determination of the skin irritation potential of biocidal products ensures that the product label contains appropriate warnings and the necessary protective measures are taken.<\/span><\/p><p><span style=\"color: #000000;\"><!-- H2-&#220;berschrift --><\/span><\/p><h2><span style=\"color: #000000;\">Methods for skin irritation tests<\/span><\/h2><p><span style=\"color: #000000;\">There are two internationally recognized basic approaches for assessing skin irritation in biocidal products: in-vivo (living animals) and in-vitro (cell culture) methods. In accordance with the 3R principles (replacement, reduction, refinement), in vitro methods are increasingly preferred.<\/span><\/p><p><span style=\"color: #000000;\"><!-- H3-Unter&#252;berschrift --><\/span><\/p><h3><span style=\"color: #000000;\">1. In vivo skin irritation test (OECD TG 404)<\/span><\/h3><p><span style=\"color: #000000;\">The OECD Test Guide 404 defines the standard procedure for the acute skin irritation\/corrosion test. With this method, the test substance is applied to the shaved skin of healthy rabbits. After 4 hours of contact time, the substance is removed and after 24, 48 and 72 hours, the skin reactions (erythema and edema) are evaluated on a scale from 0 to 4.<\/span><\/p><p><span style=\"color: #000000;\">As part of the OECD TG 404, it is first evaluated whether the test substance is corrosive. If the substance is corrosive, there is no need for an irritation test. This is a step that prevents unnecessary animal testing.<\/span><\/p><p><span style=\"color: #000000;\"><!-- H3-Unter&#252;berschrift --><\/span><\/p><h3><span style=\"color: #000000;\">2. In vitro skin irritation tests (OECD TG 439)<\/span><\/h3><p><span style=\"color: #000000;\">The modern approach gives priority to in vitro models to minimize the use of animals. OECD TG 439 is a validated test guide for in vitro skin irritation. This method uses three-dimensional tissue models (RHE) made from human skin cells.<\/span><\/p><ul><li><span style=\"color: #000000;\"><strong>EpiDerm\u2122, EpiSkin\u2122, SkinEthic\u2122:<\/strong> Kommerziell erh\u00e4ltliche, validierte menschliche Haut\u00e4quivalente.<\/span><\/li><li><span style=\"color: #000000;\"><strong>Test procedure:<\/strong> Die Testsubstanz wird 18\u201324 Stunden lang auf das Gewebe aufgetragen, anschlie\u00dfend wird die Zellviabilit\u00e4t mittels MTT-Test gemessen.<\/span><\/li><li><span style=\"color: #000000;\"><strong>Auswertung:<\/strong> Ein Viabilit\u00e4tsverlust von mehr als 50 % wird als irritierend eingestuft.<\/span><\/li><\/ul><p><span style=\"color: #000000;\">In vitro methods are increasingly being used in the routine evaluation of biocidal products due to the renunciation of animal experiments, the rapid delivery of results and reproducibility.<\/span><\/p><p><span style=\"color: #000000;\"><!-- H3-Unter&#252;berschrift --><\/span><\/p><h3><span style=\"color: #000000;\">3. Corrosion test (OECD TG 431 and TG 435)<\/span><\/h3><p><span style=\"color: #000000;\">The corrosion potential is a parameter that must be checked before the skin irritation is evaluated. Corrosive substances are determined using the OECD TG 431 (in-vitro skin corrosion test) and the OECD TG 435 (in vitro membrane barrier test). No irritation test is performed on substances that have been found to be corrosion.<\/span><\/p><p><span style=\"color: #000000;\"><!-- Tabellenanfang --><\/span><\/p><h2><span style=\"color: #000000;\">Comparison of test methods for skin irritation in biocides<\/span><\/h2><p><span style=\"color: #000000;\">The following table summarizes the methods used in evaluating the skin irritation of biocidal products:<\/span><\/p><table><thead><tr><th><span style=\"color: #000000;\">Test method<\/span><\/th><th><span style=\"color: #000000;\">Model<\/span><\/th><th><span style=\"color: #000000;\">Application time<\/span><\/th><th><span style=\"color: #000000;\">Evaluation criterion<\/span><\/th><th><span style=\"color: #000000;\">Advantages<\/span><\/th><\/tr><\/thead><tbody><tr><td><span style=\"color: #000000;\"><strong>OECD TG 404 (in vivo)<\/strong><\/span><\/td><td><span style=\"color: #000000;\">Rabbit<\/span><\/td><td><span style=\"color: #000000;\">4 Hours contact + 72 hours observation<\/span><\/td><td><span style=\"color: #000000;\">Erythem\/edema score (0\u20134)<\/span><\/td><td><span style=\"color: #000000;\">Clinical data, official recognition<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong>OECD TG 439 (in vitro)<\/strong><\/span><\/td><td><span style=\"color: #000000;\">Human Skin Equivalent (RHE)<\/span><\/td><td><span style=\"color: #000000;\">18-24 Hours<\/span><\/td><td><span style=\"color: #000000;\">Cell viability (MTT)<\/span><\/td><td><span style=\"color: #000000;\">No animal experiments, fast, reproducible<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong>OECD TG 431 (corrosive effect)<\/strong><\/span><\/td><td><span style=\"color: #000000;\">Human skin equivalent<\/span><\/td><td><span style=\"color: #000000;\">3 Min, 1 hour, 4 hours<\/span><\/td><td><span style=\"color: #000000;\">Cell viability (&amp;lt;35% = corrosion)<\/span><\/td><td><span style=\"color: #000000;\">Corrosion proof, no animal experiments<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong>OECD TG 435 (membrane barrier)<\/strong><\/span><\/td><td><span style=\"color: #000000;\">Artificial membrane<\/span><\/td><td><span style=\"color: #000000;\">For a short time<\/span><\/td><td><span style=\"color: #000000;\">Membrane damage<\/span><\/td><td><span style=\"color: #000000;\">Fast pre-screening, corrosion detection<\/span><\/td><\/tr><\/tbody><\/table><p><span style=\"color: #000000;\"><!-- Ende der Tabelle --><\/span><\/p><p><span style=\"color: #000000;\"><!-- H2-&#220;berschrift --><\/span><\/p><h2><span style=\"color: #000000;\">Skin irritation test under the BPR and national legislation<\/span><\/h2><p><span style=\"color: #000000;\">In the context of BPR (528\/2012), a separate skin irritation assessment for the active substance and the finished product must be carried out for the approval of a biocidal product. These assessments must comply with the ECHA's guidelines and OECD testing guidelines.<\/span><\/p><p><span style=\"color: #000000;\">In the approval dossier for the active ingredient, the skin irritation potential of the active ingredient itself is evaluated. In the product approval dossier, on the other hand, the skin irritation potential of the end product (active ingredient + auxiliary substances) is evaluated. In particular, the contribution of additives such as surfactants, solvents and pH regulators to the risk of irritation must be taken into account.<\/span><\/p><div class=\"note\"><span style=\"color: #000000;\"><strong>Wichtig:<\/strong> Hautirritationstests f\u00fcr Biozidprodukte m\u00fcssen in Laboren durchgef\u00fchrt werden, die den GLP-Grunds\u00e4tzen (Good Laboratory Practice) entsprechen. Andernfalls werden die erzielten Ergebnisse von den Zulassungsbeh\u00f6rden nicht anerkannt.<\/span><\/div><p><span style=\"color: #000000;\"><!-- H2-&#220;berschrift: Nano-Test --><\/span><\/p><h2><span style=\"color: #000000;\">GLP-compliant skin irritation tests for biocidal products with nano-test<\/span><\/h2><p><span style=\"color: #000000;\">Hautirritationstests bilden f\u00fcr Hersteller von Biozidprodukten die Grundlage sowohl f\u00fcr das Zulassungsverfahren als auch f\u00fcr die Bewertung der Produktsicherheit. <strong>Nano-Test<\/strong> ist mit seinen GLP-konformen Analysedienstleistungen in diesem Bereich ein zuverl\u00e4ssiger L\u00f6sungspartner f\u00fcr die Biozidbranche.<\/span><\/p><div class=\"highlight\"><h3><span style=\"color: #000000;\">Nano-Test \u2013 Performance Description<\/span><\/h3><p><span style=\"color: #000000;\">Nano-Test bietet f\u00fcr Biozidprodukte <strong>Hautirritationstests (Skin Irritation Test)<\/strong> an. In unseren nach GLP und ISO\/IEC 17025 akkreditierten Laboren werden sowohl <strong>in vivo (OECD TG 404)<\/strong> als auch <strong>in vitro (OECD TG 439, TG 431, TG 435)<\/strong> Methoden angewendet.<\/span><\/p><p><span style=\"color: #000000;\">Our service portfolio:<\/span><\/p><ul><li><span style=\"color: #000000;\"><strong>In-vitro-Hautirritationstest (OECD TG 439):<\/strong> Messung der Zellviabilit\u00e4t mit RHE-Modellen, schnelle Ergebnisse ohne Tierversuche.<\/span><\/li><li><span style=\"color: #000000;\"><strong>In-vivo-Hautirritationstest (OECD TG 404):<\/strong> Bewertung von Erythem und \u00d6dem am Kaninchenmodell, von den Aufsichtsbeh\u00f6rden anerkannte klinische Daten.<\/span><\/li><li><span style=\"color: #000000;\"><strong>Korrosionstests (OECD TG 431\/435):<\/strong> Identifizierung korrosiver Stoffe, Vermeidung unn\u00f6tiger Reizungstests.<\/span><\/li><li><span style=\"color: #000000;\"><strong>BPR-konforme Berichterstattung:<\/strong> Berichte in voller \u00dcbereinstimmung mit den ECHA-Leitf\u00e4den und den BPR-Anh\u00e4ngen.<\/span><\/li><li><span style=\"color: #000000;\"><strong>Methodenvalidierung:<\/strong> Entwicklung und Validierung von Methoden, die speziell auf Ihre Produktmatrix zugeschnitten sind.<\/span><\/li><\/ul><p><span style=\"color: #000000;\">Alle unsere Analysen werden als <strong>GLP-konforme Berichte<\/strong> vorgelegt, die \u00fcber eine Inspektionsgenehmigung verf\u00fcgen und die Datenintegrit\u00e4t (ALCOA+-Prinzipien) gew\u00e4hrleisten.<\/span><\/p><\/div><h3><span style=\"color: #000000;\">Advantages of nano-test in the biocide skin irritation test:<\/span><\/h3><ul><li><span style=\"color: #000000;\"><strong>Kompetenz in beiden Methoden:<\/strong> Sowohl In-vivo- als auch In-vitro-Irritationstests an einem Standort.<\/span><\/li><li><span style=\"color: #000000;\"><strong>BPR-Konformit\u00e4t:<\/strong> Vollst\u00e4ndige \u00dcbereinstimmung mit den ECHA-Leitf\u00e4den und den BPR-Anforderungen.<\/span><\/li><li><span style=\"color: #000000;\"><strong>3R-Prinzipien:<\/strong> In vitro-Methoden werden, wann immer m\u00f6glich, bevorzugt.<\/span><\/li><li><span style=\"color: #000000;\"><strong>Schnelle Ergebnisse:<\/strong> Berichterstattung bei In-vitro-Tests innerhalb von 3\u20135 Werktagen.<\/span><\/li><li><span style=\"color: #000000;\"><strong>Fachkompetentes Team:<\/strong> Ein Team mit Erfahrung in den Bereichen Toxikologie, Biozidrecht und analytische Chemie.<\/span><\/li><\/ul><div class=\"note\"><span style=\"color: #000000;\"><strong>Note:<\/strong> Der Hautirritationstest ist nur ein Teil der Sicherheitsbewertung eines Biozidprodukts. Er sollte zusammen mit anderen Tests wie Augenirritation, Sensibilisierung und systemischer Toxizit\u00e4t ausgewertet werden. Nano-Test beschleunigt Ihren Prozess, indem es all diese Tests aus einer Hand anbietet.<\/span><\/div><p><span style=\"color: #000000;\"><!-- H2-&#220;berschrift: Ergebnis --><\/span><\/p><p><span style=\"color: #000000;\">Hautreizungen, die durch Biozidprodukte verursacht werden k\u00f6nnen, stellen sowohl hinsichtlich der beruflichen Exposition als auch der Verbrauchersicherheit ein erhebliches Risiko dar. <strong>Der Biozid-Hautirritationstest<\/strong> ist unerl\u00e4sslich, um dieses Risiko wissenschaftlich zu bewerten und die erforderlichen Warnhinweise auf der Produktkennzeichnung anzubringen. Die Auswahl einer den OECD-Pr\u00fcfrichtlinien entsprechenden Methode, die korrekte Durchf\u00fchrung und eine GLP-konforme Berichterstattung sind Voraussetzungen f\u00fcr die Genehmigung Ihres Zulassungsantrags im Rahmen der BPR.<\/span><\/p><p><span style=\"color: #000000;\">With its GLP-accredited laboratory infrastructure, its specialist staff and well-founded knowledge of the current legal regulations, NANO-TEST comprehensively meets the requirements of manufacturers of biocidal products for skin irritation tests. Collaborate with Nano-Test to increase the safety of your products and accelerate market access.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Biozidprodukte sind Zubereitungen, die aktive chemische Stoffe enthalten und in einem breiten Spektrum eingesetzt werden \u2013 von Desinfektionsmitteln \u00fcber Sch\u00e4dlingsbek\u00e4mpfungsmittel bis hin zu Holzschutzmitteln und Antifouling-Farben. Je nach Verwendungszweck k\u00f6nnen diese Produkte direkt oder indirekt mit der menschlichen Haut in Kontakt kommen. Insbesondere Produkte wie Handdesinfektionsmittel, Oberfl\u00e4chenreiniger und Pflanzenschutzmittel k\u00f6nnen potenziell hautreizend wirken. Daher ist \u2026 <a href=\"https:\/\/nano-test.de\/en\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/\" class=\"more-link\" title=\"Continue reading &amp;lt;span class=\" screen-reader-text>Hautreizungstest durch Biozide | OECD 404\u201c&gt;Continue reading <span class=\"screen-reader-text\">Skin irritation test by biocides | OECD 404<\/span><\/a><\/p>\n","protected":false},"author":4,"featured_media":4560,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-4559","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biozid-blog"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Hautreizungstest durch Biozide | OECD 404 | Nano-Test\u00ae<\/title>\n<meta name=\"description\" content=\"Hautirritationstest f\u00fcr Biozidprodukte: OECD-Testrichtlinie 404, In-vitro-RHE-Verfahren und Anforderungen der Biozid-Verordnung (BPR) 528\/2012. GLP-konforme Dienstleistungen von Nano-Test zur Analyse der Hautirritation durch Biozide.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Hautreizungstest durch Biozide | OECD 404 | Nano-Test\u00ae\" \/>\n<meta property=\"og:description\" content=\"Hautirritationstest f\u00fcr Biozidprodukte: OECD-Testrichtlinie 404, In-vitro-RHE-Verfahren und Anforderungen der Biozid-Verordnung (BPR) 528\/2012. GLP-konforme Dienstleistungen von Nano-Test zur Analyse der Hautirritation durch Biozide.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/\" \/>\n<meta property=\"og:site_name\" content=\"Nano-Test\u00ae\" \/>\n<meta property=\"article:published_time\" content=\"2026-10-12T20:04:01+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-10-12T20:05:12+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/nano-test.de\/wp-content\/uploads\/2026\/10\/Hautreizungstest-durch-Biozide-OECD-404.png\" \/>\n\t<meta property=\"og:image:width\" content=\"750\" \/>\n\t<meta property=\"og:image:height\" content=\"450\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Sultan Culum\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Sultan Culum\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"7 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/\"},\"author\":{\"name\":\"Sultan Culum\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/#\\\/schema\\\/person\\\/e873a4aee4023b424d94f10e41a12670\"},\"headline\":\"Hautreizungstest durch Biozide | OECD 404\",\"datePublished\":\"2026-10-12T20:04:01+00:00\",\"dateModified\":\"2026-10-12T20:05:12+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/\"},\"wordCount\":1176,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/nano-test.de\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/Hautreizungstest-durch-Biozide-OECD-404.png\",\"articleSection\":[\"Biozid\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/\",\"url\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/\",\"name\":\"Hautreizungstest durch Biozide | OECD 404 | Nano-Test\u00ae\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/nano-test.de\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/Hautreizungstest-durch-Biozide-OECD-404.png\",\"datePublished\":\"2026-10-12T20:04:01+00:00\",\"dateModified\":\"2026-10-12T20:05:12+00:00\",\"description\":\"Hautirritationstest f\u00fcr Biozidprodukte: OECD-Testrichtlinie 404, In-vitro-RHE-Verfahren und Anforderungen der Biozid-Verordnung (BPR) 528\\\/2012. GLP-konforme Dienstleistungen von Nano-Test zur Analyse der Hautirritation durch Biozide.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/#primaryimage\",\"url\":\"https:\\\/\\\/nano-test.de\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/Hautreizungstest-durch-Biozide-OECD-404.png\",\"contentUrl\":\"https:\\\/\\\/nano-test.de\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/Hautreizungstest-durch-Biozide-OECD-404.png\",\"width\":750,\"height\":450},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/2026\\\/10\\\/12\\\/hautreizungstest-durch-biozide-oecd-404\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/nano-test.de\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Hautreizungstest durch Biozide | OECD 404\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/#website\",\"url\":\"https:\\\/\\\/nano-test.de\\\/\",\"name\":\"NANO-TEST\u00ae\",\"description\":\"Laboranalysen, Testservices, Pr\u00fcflabor &amp; Qualit\u00e4tskontrollen in Deutschland\",\"publisher\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/nano-test.de\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/#organization\",\"name\":\"NANO-TEST\u00ae\",\"url\":\"https:\\\/\\\/nano-test.de\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/nano-test.de\\\/wp-content\\\/uploads\\\/2026\\\/03\\\/Nanotest-Logo-NT-scaled.png\",\"contentUrl\":\"https:\\\/\\\/nano-test.de\\\/wp-content\\\/uploads\\\/2026\\\/03\\\/Nanotest-Logo-NT-scaled.png\",\"width\":2510,\"height\":2560,\"caption\":\"NANO-TEST\u00ae\"},\"image\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/#\\\/schema\\\/logo\\\/image\\\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/#\\\/schema\\\/person\\\/e873a4aee4023b424d94f10e41a12670\",\"name\":\"Sultan Culum\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/ef47288e2a0dc3ff55e71e172f39c9c82ffbf55bad226f89d1aaa823e517a73b?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/ef47288e2a0dc3ff55e71e172f39c9c82ffbf55bad226f89d1aaa823e517a73b?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/ef47288e2a0dc3ff55e71e172f39c9c82ffbf55bad226f89d1aaa823e517a73b?s=96&d=mm&r=g\",\"caption\":\"Sultan Culum\"},\"sameAs\":[\"https:\\\/\\\/nano-test.de\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Skin irritation test by biocides | OECD 404 | Nano-Test\u00ae","description":"Skin irritation test for biocidal products: OECD Test Guideline 404, in vitro rhe methods and requirements of the Biocide Regulation (BPR) 528\/2012. GLP-compliant nano-test services to analyze skin irritation by biocides.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/","og_locale":"en_US","og_type":"article","og_title":"Hautreizungstest durch Biozide | OECD 404 | Nano-Test\u00ae","og_description":"Hautirritationstest f\u00fcr Biozidprodukte: OECD-Testrichtlinie 404, In-vitro-RHE-Verfahren und Anforderungen der Biozid-Verordnung (BPR) 528\/2012. GLP-konforme Dienstleistungen von Nano-Test zur Analyse der Hautirritation durch Biozide.","og_url":"https:\/\/nano-test.de\/en\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/","og_site_name":"Nano-Test\u00ae","article_published_time":"2026-10-12T20:04:01+00:00","article_modified_time":"2026-10-12T20:05:12+00:00","og_image":[{"width":750,"height":450,"url":"https:\/\/nano-test.de\/wp-content\/uploads\/2026\/10\/Hautreizungstest-durch-Biozide-OECD-404.png","type":"image\/png"}],"author":"Sultan Culum","twitter_card":"summary_large_image","twitter_misc":{"Written by":"Sultan Culum","Est. reading time":"7 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/#article","isPartOf":{"@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/"},"author":{"name":"Sultan Culum","@id":"https:\/\/nano-test.de\/#\/schema\/person\/e873a4aee4023b424d94f10e41a12670"},"headline":"Hautreizungstest durch Biozide | OECD 404","datePublished":"2026-10-12T20:04:01+00:00","dateModified":"2026-10-12T20:05:12+00:00","mainEntityOfPage":{"@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/"},"wordCount":1176,"commentCount":0,"publisher":{"@id":"https:\/\/nano-test.de\/#organization"},"image":{"@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/#primaryimage"},"thumbnailUrl":"https:\/\/nano-test.de\/wp-content\/uploads\/2026\/10\/Hautreizungstest-durch-Biozide-OECD-404.png","articleSection":["Biozid"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/","url":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/","name":"Skin irritation test by biocides | OECD 404 | Nano-Test\u00ae","isPartOf":{"@id":"https:\/\/nano-test.de\/#website"},"primaryImageOfPage":{"@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/#primaryimage"},"image":{"@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/#primaryimage"},"thumbnailUrl":"https:\/\/nano-test.de\/wp-content\/uploads\/2026\/10\/Hautreizungstest-durch-Biozide-OECD-404.png","datePublished":"2026-10-12T20:04:01+00:00","dateModified":"2026-10-12T20:05:12+00:00","description":"Skin irritation test for biocidal products: OECD Test Guideline 404, in vitro rhe methods and requirements of the Biocide Regulation (BPR) 528\/2012. GLP-compliant nano-test services to analyze skin irritation by biocides.","breadcrumb":{"@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/#primaryimage","url":"https:\/\/nano-test.de\/wp-content\/uploads\/2026\/10\/Hautreizungstest-durch-Biozide-OECD-404.png","contentUrl":"https:\/\/nano-test.de\/wp-content\/uploads\/2026\/10\/Hautreizungstest-durch-Biozide-OECD-404.png","width":750,"height":450},{"@type":"BreadcrumbList","@id":"https:\/\/nano-test.de\/2026\/10\/12\/hautreizungstest-durch-biozide-oecd-404\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/nano-test.de\/"},{"@type":"ListItem","position":2,"name":"Skin irritation test by biocides | OECD 404"}]},{"@type":"WebSite","@id":"https:\/\/nano-test.de\/#website","url":"https:\/\/nano-test.de\/","name":"NANO-TEST\u00ae","description":"Laboratory analyses, testing services, testing laboratories and quality control in Germany","publisher":{"@id":"https:\/\/nano-test.de\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/nano-test.de\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/nano-test.de\/#organization","name":"NANO-TEST\u00ae","url":"https:\/\/nano-test.de\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/nano-test.de\/#\/schema\/logo\/image\/","url":"https:\/\/nano-test.de\/wp-content\/uploads\/2026\/03\/Nanotest-Logo-NT-scaled.png","contentUrl":"https:\/\/nano-test.de\/wp-content\/uploads\/2026\/03\/Nanotest-Logo-NT-scaled.png","width":2510,"height":2560,"caption":"NANO-TEST\u00ae"},"image":{"@id":"https:\/\/nano-test.de\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/nano-test.de\/#\/schema\/person\/e873a4aee4023b424d94f10e41a12670","name":"Sultan Culum","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/ef47288e2a0dc3ff55e71e172f39c9c82ffbf55bad226f89d1aaa823e517a73b?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/ef47288e2a0dc3ff55e71e172f39c9c82ffbf55bad226f89d1aaa823e517a73b?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/ef47288e2a0dc3ff55e71e172f39c9c82ffbf55bad226f89d1aaa823e517a73b?s=96&d=mm&r=g","caption":"Sultan Culum"},"sameAs":["https:\/\/nano-test.de"]}]}},"_links":{"self":[{"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/posts\/4559","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/comments?post=4559"}],"version-history":[{"count":0,"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/posts\/4559\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/media\/4560"}],"wp:attachment":[{"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/media?parent=4559"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/categories?post=4559"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nano-test.de\/wp-json\/wp\/v2\/tags?post=4559"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}