{"id":2247,"date":"2025-06-23T14:42:41","date_gmt":"2025-06-23T14:42:41","guid":{"rendered":"https:\/\/nano-test.de\/?p=2247"},"modified":"2025-07-01T11:10:58","modified_gmt":"2025-07-01T11:10:58","slug":"oecd-474-mammalian-eritrosit-mikronukleustest","status":"publish","type":"post","link":"https:\/\/nano-test.de\/en\/2025\/06\/23\/oecd-474-mammalian-eritrosit-mikronukleustest\/","title":{"rendered":"OECD Test Guideline 474 &#8211; Mammalian Eritrosite"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2247\" class=\"elementor elementor-2247\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-24a635ae ts-col-stretched-none ts-bg-color-over-image elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"24a635ae\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5b0f00ff ts-bg-color-over-image\" data-id=\"5b0f00ff\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-62503973 elementor-widget elementor-widget-text-editor\" data-id=\"62503973\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>OECD Test Guideline 474: What is the Mammalian Erythrocyte Micronucleus Test?<\/h2><p>Zur Bewertung der Sicherheit neu entwickelter pharmazeutischer, biozider oder anderer chemischer Produkte f\u00fcr die Gesundheit von Mensch und Umwelt werden verschiedene toxikologische Tests durchgef\u00fchrt. Einer dieser Tests ist die OECD-Testrichtlinie 474<b>\u00a0\u2013 S\u00e4ugetier-Erythrozyten-Mikronukleus-Test<\/b>.<\/p><p>Dieser im Rahmen der <a href=\"https:\/\/nano-test.de\/en\/service\/gute-laborpraxis-glp\/\">GLP (Gute Laborpraxis)<\/a> durchgef\u00fchrte Test wird insbesondere verwendet, um das Potenzial von Produkten zur Sch\u00e4digung des genetischen Materials, d. h. das <b>Genotoxizit\u00e4tsrisiko<\/b>, aufzuzeigen.<\/p><h3>Purpose of Mammalian Erythrocyte Micronucleus Test<\/h3><p>This test examines the formation of micronuclei (small, abnormal nuclear structures) in erythrocytes from bone marrow or peripheral blood to detect chromosomal damage. Micronuclei are formed by the incorrect separation of chromosomes or chromosome fragments during cell division and are considered an indicator of genetic damage.<\/p><ul><li>This test is particularly preferred in the following cases:<\/li><li>Development process of pharmaceutical products<\/li><li>Safety assessments of biocidal and pesticide products<\/li><li>Toxicological Testing of Industrial Chemicals<\/li><li>Required cases for cosmetics or personal care products<\/li><\/ul><h3>Application process according to OECD TG 474<\/h3><p>The micronucleus test performed in accordance with GLP standards includes the following steps:<\/p><ol><li><b>Vorbereitung der zu testenden Substanz:<\/b>Das Produkt wird unter Festlegung geeigneter L\u00f6sungsmittel und Dosierungsbereiche vorbereitet.<\/li><li><b>Verwendung von Versuchstieren:<\/b>In der Regel werden M\u00e4use oder Ratten verwendet. Vor Beginn der Arbeit wird die Genehmigung der Ethikkommission eingeholt.<\/li><li><b>Exposition:<\/b> Die Testsubstanz wird den Tieren oral, dermal oder durch Inhalation verabreicht.<\/li><li><b>Probenahme:<\/b> Nach einer bestimmten Zeit werden Erythrozytenproben aus dem Knochenmark oder peripherem Blut entnommen.<\/li><li><b>Mikronukleus-Analyse:<\/b> Die Erythrozyten werden unter dem Mikroskop untersucht. Die H\u00e4ufigkeit der Mikronukleusbildung wird bestimmt.<\/li><li><b>Auswertung der Ergebnisse:<\/b> Es wird ein Vergleich mit der Kontrollgruppe durchgef\u00fchrt und eine statistische Analyse vorgenommen.<\/li><\/ol><h3>For which products is the erythrocyte micronucleus test in mammals suitable?<\/h3><ul><li><a href=\"https:\/\/nano-test.de\/en\/service\/pharma\/\">Pharmazeutische<\/a> Rohstoffe und Fertigprodukte<\/li><li><a href=\"https:\/\/nano-test.de\/en\/service\/biozid\/\">Biocidal products<\/a><\/li><li>Pesticides and agricultural chemicals<\/li><li>Industrial Chemicals<\/li><li><a href=\"https:\/\/nano-test.de\/en\/service\/kosmetik\/\">Kosmetika<\/a> und K\u00f6rperpflegeprodukte<\/li><li><a href=\"https:\/\/nano-test.de\/en\/service\/lebensmittelanalysen\/\">Lebensmittelzusatzstoffe<\/a> (falls erforderlich)<\/li><\/ul><h3>Why choose our laboratory?<\/h3><ul><li>GLP accreditation and OECD TG 474 compliant test infrastructure<\/li><li>Expert toxicologists and experienced laboratory team<\/li><li>Working in accordance with ethical principles and the 3R principles<\/li><li>Fast, reliable and compliant reporting<\/li><li>Scientific support in approval and R&amp;D processes<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>OECD-Testrichtlinie 474: Was ist der S\u00e4ugetier-Erythrozyten-Mikronukleus-Test? Zur Bewertung der Sicherheit neu entwickelter pharmazeutischer, biozider oder anderer chemischer Produkte f\u00fcr die Gesundheit von Mensch und Umwelt werden verschiedene toxikologische Tests durchgef\u00fchrt. Einer dieser Tests ist die OECD-Testrichtlinie 474\u00a0\u2013 S\u00e4ugetier-Erythrozyten-Mikronukleus-Test. Dieser im Rahmen der GLP (Gute Laborpraxis) durchgef\u00fchrte Test wird insbesondere verwendet, um das Potenzial von Produkten \u2026 <a href=\"https:\/\/nano-test.de\/en\/2025\/06\/23\/oecd-474-mammalian-eritrosit-mikronukleustest\/\" class=\"more-link\" title=\"Continue reading &amp;lt;span class=\" screen-reader-text>OECD-Testrichtlinie 474 \u2013 Mammalian-Eritrosit\u201c&gt;Continue reading <span class=\"screen-reader-text\">OECD Test Guideline 474 \u2013 Mammalian Eritrosite<\/span><\/a><\/p>\n","protected":false},"author":4,"featured_media":2248,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-2247","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-glp-blog"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>OECD 474 - Mammalian-Eritrosit-Mikronukleustest | GLP | Nano-Test\u00ae<\/title>\n<meta name=\"description\" content=\"Bewertung der Genotoxizit\u00e4t in pharmazeutischen und chemischen Produkten mit dem OECD 474 S\u00e4ugetier-Erythrozyten-Mikronukleustest.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nano-test.de\/2025\/06\/23\/oecd-474-mammalian-eritrosit-mikronukleustest\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"OECD 474 - Mammalian-Eritrosit-Mikronukleustest | GLP | Nano-Test\u00ae\" \/>\n<meta property=\"og:description\" content=\"Bewertung der Genotoxizit\u00e4t in pharmazeutischen und chemischen Produkten mit dem OECD 474 S\u00e4ugetier-Erythrozyten-Mikronukleustest.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/nano-test.de\/2025\/06\/23\/oecd-474-mammalian-eritrosit-mikronukleustest\/\" \/>\n<meta property=\"og:site_name\" content=\"Nano-Test\u00ae\" \/>\n<meta property=\"article:published_time\" content=\"2025-06-23T14:42:41+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-07-01T11:10:58+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/nano-test.de\/wp-content\/uploads\/2025\/06\/OECD-TG-474-Memel-Eritrosit-Mikronukleustest.png\" \/>\n\t<meta property=\"og:image:width\" content=\"2560\" \/>\n\t<meta property=\"og:image:height\" content=\"1707\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Sultan Culum\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Sultan Culum\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/2025\\\/06\\\/23\\\/oecd-474-mammalian-eritrosit-mikronukleustest\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nano-test.de\\\/2025\\\/06\\\/23\\\/oecd-474-mammalian-eritrosit-mikronukleustest\\\/\"},\"author\":{\"name\":\"Sultan Culum\",\"@id\":\"https:\\\/\\\/nano-test.de\\\/#\\\/schema\\\/person\\\/e873a4aee4023b424d94f10e41a12670\"},\"headline\":\"OECD-Testrichtlinie 474 &#8211; 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